The MRL is set up to investigate various aspects of mitochondrial function and disease from different approaches and is therefore a truly multidisciplinary research group. 

The laboratory is a very well-equipped with specialised apparatus for the following:

• Mitochondrial function:

  • Mitochondrial oxidative phosphorylation and glycolysis using a Seahorse XF96e analyser;  

  • Mitochondrial metabolomics;

  • High throughput mitochondrial enzyme kinetics;

  • Cellular imaging;

  • Oxidative stress evaluations.

• Molecular biology and protein analyses:

  • All basic molecular biology techniques (PCR /real-time PCR, knockdown, recombinant DNA etc.);

  • Next generation sequencing;

  • mtDNA investigations;

  • All basic electrophoresis and immuno-based protein analyses and quantification;

  • Enzyme kinetics.

• Cell/tissue culture facility:

  • All basic cell/tissue culture techniques.

• Associated laboratories and capacities:

  • In-house Ion-Torrent platform (Ion S5/Ion Chef workflow) and bioinformatics.

  • In-house Metabolomics Analytical Platform for targeted and untargeted metabolomics and bioinformatics;

  • An accredited Animal Research Centre on campus where our mouse models are housed.