We mainly use “high end instruments” for untargeted analysis. Because untargeted analysis makes use of little (or even no) sample preparation, these instruments must be highly selective in order to separate signals from closely related compounds (in terms of mass, retention time or fragmentation spectra). The high end instruments used at our institution are:

  • GCxGC-TOFMS (to cover primary metabolites)
  • LC-IM-QTOFMS (to cover secondary metabolites)
  • NMR (to cover highly abundant metabolites)

The NMP mainly use so called “low end instruments” for targeted analysis. With targeted analysis, the compound of interest is selectively extracted/isolated from the sample matrix and selectively quantified using its unique retention time, mass, electromagnetic absorbance and/or magnetic resonance characteristics. The low end instruments used at our institution are:

  • LC-MS-MS
  • GC-MS
  • HPLC (UV/vis detection)
  • Ion Trap
  • CE (UV/vis detection)
  • NMR

Our semi-targeted techniques make use of both high- and low end instruments to selectively analyse metabolite sets. These include:

  • LC-MS-MS
  • GC-MS