The Bruker MicroQTOF II is a high-resolution mass spectrometer that is equipped with both DIP-APCI and ESI ionization sources. This instrument is widely used in various research fields, such as metabolomics, proteomics, and environmental analysis, due to its high sensitivity, accuracy, and resolution. The DIP-APCI source allows for the direct injection of samples without the need for chromatographic separation, while the ESI source provides excellent ionization efficiency for polar compounds. The MicroQTOF II can detect and analyze molecules with a mass range of up to 50,000 Da, making it a versatile instrument for a wide range of applications. The data obtained from this instrument can be processed using various software packages for qualitative and quantitative analysis of complex samples.